Rational Design of a Dual Mutant Degrading Enzyme with Improved Catalytic Efficiency and Acid Tolerance for Four Aflatoxins.
Aflatoxins are major contaminants in raw food materials and their products. Developing enzymes that efficiently degrade multiple aflatoxins under acidic conditions has great practical value. In this study, the aflatoxin-degrading enzyme ADPPIII from Aspergillus terreus was systematically engineered. Substrate tunnel op...