Rationally optimized split crRNA-based CRISPR/Cas12a platform for amplification-free miRNA detection.
MicroRNAs (miRNAs) are important biomarkers for cancer diagnosis and prognosis, but their short length, low abundance, and high sequence similarity make rapid and sensitive detection challenging. Split crRNA-based CRISPR/Cas12a systems have emerged as a promising amplification-free strategy for RNA detection, yet the d...