Engineering a leakage-resistant CHA-CRISPR/Cas12a biosensor via cis-cleavage-triggered split activator assembly for ultrasensitive miRNA detection.
MicroRNAs (miRNAs) have emerged as important biomarkers for cancer diagnosis and prognosis, yet their accurate detection remains challenging due to low abundance, short sequences, and sequence homology. Herein, we report a novel biosensing platform based on a cascade cis-cleavage-triggered split activator assembly stra...