Author

Qun-Xin She

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Open access Sep 2026

BaCas12a3 represents a new subtype of type V CRISPR effector with collateral activity toward tRNA

Abstract The CRISPR–Cas12 family encompasses diverse RNA-guided nucleases with both DNA- and RNA-targeting subtypes. They can trigger antiviral activities through either direct elimination of invading nucleic acids or activating broad collateral cleavage to induce abortive infection. Here, we report a novel type V CRIS...

Xin Li, Chen-Wei Wu, Jin-Shan Guo et al. · Nucleic Acids Research · 0 citations

Open access Aug 2026

Recruitment of Cas3 enables DNA cleavage by the type I-A CRISPR-Cas system of Saccharolobus islandicus.

This study isolates the I-A Cascade from Saccharolobus islandicus, revealing a minimal form of Cascade lacking both Cas3 and the CRISPR-RNA maturase Cas6, and shows that ATP not only enables the processive target degradation by Cas3 but also suppresses the trans-cleavage of the same enzyme.

Su-Ping Jiang, Xuhui Tian, Fang Wang et al. · Cell Reports · 1 citation