Establishing wastewater metagenomics as a quantitative pathogen monitoring tool with normalization
Wastewater metagenomic sequencing (WW-MGS) enables simultaneous detection of hundreds of pathogens, but its use for quantitative pathogen tracking has not been robustly validated. Like wastewater PCR (WW-PCR), WW-MGS is affected by biases from variable fecal dilution and sample processing, but must additionally contend...