Basal Cell Expansion Conditions Influence Differentiation and Phenotypic Outcomes in Primary Nasal Epithelia Grown at Air–Liquid Interface
Background/Objectives: Primary airway epithelia grown at air–liquid interface (ALI) are widely used to model respiratory endophenotypes and to enable individualized functional testing. However, culture conditions used to establish ALI vary considerably and may introduce selection biases. We aimed to adapt our lung expl...