Genetically encoded bioluminescent calcium indicators enable excitation-free imaging of Ca2+ dynamics with low background and without excitation-associated photobleaching. However, many exhibit lower dynamic ranges than their fluorescent counterparts, motivating the development of new sensor configurations, particularl...
Protein engineering appears to be entering a golden age, defined by a rapidly accelerating pace of progress, even as significant challenges in design, screening, and real-world application remain.
H. Ai, Frances H. Arnold, Doug Barrick et al.· Protein engineering, design...· 0 citations
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