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Sponsored AI Networking Cookbook: Practical recipes for AI-assisted network automation and development AI Networking Cookbook: Practical recipes for AI-assisted network automation and development

Author

Rakhi Rajan

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Open access Sep 2026

Active-site arginines differentially control Cas12a DNA cleavage and specificity.

It is established that a positive charge at 921 is required for CRISPR RNA-dependent DNA cleavage (cis cleavage), whereas R918 primarily enhances cleavage efficiency, and the potential of modifying active pocket residues to reduce unwanted DNA cleavage while increasing on-target specificity is highlighted.

Saadi Rostami, A. M. dos Santos, Richard S. Van et al. · 0 citations

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