Biochemical characterization and assay development for adenylosuccinate synthetase Rv0357c from Mycobacterium tuberculosis.
The expression, purification, and enzymatic characterization of recombinant Mtb ADSS are reported, providing the first comprehensive biochemical framework for studying Mtb ADSS and establishing a foundation for structure-guided inhibitor discovery targeting purine biosynthesis as a novel antitubercular strategy.