One-pot RPA-CRISPR/Cas12a assay for rapid and sensitive detection of Sclerotium rolfsii in Glycine max
Soil-borne fungal diseases are major constraints to the yield of legume crops, and it is therefore urgent to develop rapid and accurate detection methods for plant pathogens. In this study, a one-pot fluorescent detection system combining recombinase polymerase amplification (RPA) with CRISPR/Cas12a was established f...