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Author

Roquyya Gul

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Open access Sep 2026

Prokaryotic expression and characterization of IL-21 and its combined effect with IL-2 as an efficacious chimeric drug for cancer therapy

Human interleukin-2 (IL-2) and interleukin-21 (IL-21) are pleiotropic cytokines crucial for immune cell activation and proliferation. This study reports the cloning, expression, and characterization of IL-21 in E. coli system using the pET28a (+) vector. Optimal induction with 0.1 mM IPTG yielded ~ 40% IL-21 expr...

Qurrat Ul Ain Shafique, Hanfa Ashfaq, A. Tayyeb et al. · 0 citations

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