Generation of spCAS9 expressing human mesenchymal stem cell line to study gene function during osteoblast differentiation
Abstract Human bone marrow-derived stromal cells (hMSCs) are a great resource for studying how genes influence cell fate and differentiation into various cell types like osteoblasts, adipocytes, and chondrocytes, among other cell types. However, genetic manipulation of primary hMSCs has been challenging due to their sh...