Author

Ruilin Tian

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Open access Jul 2026

A pooled CRISPR-based neuronal activity screen links TMEM50A-dependent MVB function to synaptic integrity and remote memory.

A high-throughput functional genomics platform that couples the calcium-integrating sensor CaMPARI2 with CRISPRi screening in human iPSC-derived neurons is established and reveals a critical role for TMEM50A-dependent MVB function in maintaining synaptic integrity and behavior.

Jian-Hui Wang, Meiqi Liu, Yi-Ming Chen et al. · Cell Reports · 0 citations

Open access Aug 2026

A massively parallel CRISPR-based screening platform for modifiers of neuronal depolarization

A platform to uncover genes affecting neuronal excitability in scalable CRISPR screens using CRISPRi and the fluorescent calcium integrator CaMPARI2 is developed to uncover molecular mechanisms controlling neuronal function in health and disease.

Steven C. Boggess, Vaidehi Gandhi, Ming-Chi Tsai et al. · Nature Communications · 0 citations