Photodynamic therapy (PDT) is a promising non-invasive strategy for breast cancer, yet its clinical translation is hindered by fluorescence quenching, insufficient reactive oxygen species (ROS) generation and poor tumor targeting. Herein, a novel lipid droplet-targeted aggregation-induced emission (AIE) photosensitizer CTC was designed and synthesized. CTC has a large Stokes shift of 150 nm to avoid self-absorption and exhibits typical AIE properties in aqueous environments. Under white light irradiation, it efficiently generates three cytotoxic ROS (1O2, ·OH, O2•-), with a high colocalization coefficient of 0.95 with lipid droplets in 4 T1 cells. CTC shows excellent biocompatibility in the dark and potent phototoxicity under illumination. In vivo, CTC combined with light significantly inhibits 4 T1 tumor growth without systemic toxicity. Density functional theory calculations elucidate its multi-type ROS generation mechanism, providing a novel candidate and new insight for safe and efficient breast cancer PDT.
Yukun Zhang, Lidong Deng, Qiyan Li et al.· Bioorganic chemistry (Print)· 0 citations
Thiophenol (PhSH) is a highly toxic aromatic thiol endangering ecosystems, food safety, and human health. Traditional detection methods are limited by instrument dependence and inability for real-time imaging. This study developed a red-emitting fluorescent probe, TTCP-DN, based on the PET mechanism. The probe employs 5-(4-diphenylamino)benzothiophene as the fluorophore and 2,4-dinitrophenoxy as the PhSH-specific recognition moiety, achieving mitochondrial targeting via a pyridine salt. Without PhSH, fluorescence is quenched; upon PhSH-induced reaction, fluorescence restores at 623 nm. The probe has low detection limit (37.8 nM), large Stokes shift (153 nm), good biocompatibility, and high selectivity. It was applied for mitochondrial imaging in 4 T1 cells, qualitative detection and semi-quantitative concentration trend analysis of PhSH in zebrafish, plant tissues, environmental, food and biological samples (recoveries 95-105%), and fabricated into portable paper-based test strips. This work provides a versatile PhSH detection tool and a paradigm for targeted probes of toxic aromatic pollutants.
Yukun Zhang, Xiaoli Li, Ruiyuan Liu et al.· Food Chemistry· 0 citations
ABSTRACT Diabetic wounds are characterized by a complex pathological microenvironment, marked by persistent hypoxia, excessive reactive oxygen species (ROS), chronic inflammation, and bacterial infection. To address this challenge, we developed a multifunctional composite hydrogel (CFP/PC@MnO2) for diabetic wound healing. It is formed by covalent crosslinking of carboxymethyl chitosan (CMCS), 4‐formylphenylboronic acid (4FPBA), and polyvinyl alcohol (PVA) via Schiff base and boronate ester bonds, followed by incorporation of phycocyanin‐modified MnO2 nanoparticles (PC@MnO2 NPs). The CFP/PC@MnO2 hydrogel exhibits favorable mechanical properties, local injectability, self‐healing capability, tissue adhesion, and the ability to form a protective wound barrier. Meanwhile, the CFP/PC@MnO2 hydrogel degrades rapidly under ROS, releasing PC@MnO2 to scavenge ROS and generate O2 in situ to alleviate hypoxia; the PC@MnO2 NPs further exhibit strong NIR‐triggered photothermal antibacterial activity. In vitro, this hydrogel exhibits excellent biocompatibility, effectively scavenges ROS, alleviates oxidative stress, and suppresses inflammation. In a full‐thickness wound model in diabetic rats, the CFP/PC@MnO2 hydrogel combined with photothermal therapy effectively eradicates bacteria, promotes M2 macrophage polarization to modulate the immune microenvironment, and enhances angiogenesis and collagen deposition, thereby accelerating wound healing. Overall, this multifunctional hydrogel integrates ROS scavenging, self‐sustained oxygenation, immunomodulation, and photothermal antibacterial action, offering a synergistic, innovative strategy for diabetic wound treatment.
Zhi Xu, Xiaodong Luo, Jingxia Wu et al.· Advancement of science· 0 citations
A multifunctional composite hydrogel (GelDA/OPL) with excellent adhesion, self-healing properties, injectability, and photothermal antibacterial activity through Schiff base crosslinking between dopamine-modified gelatin (GelDA) and oxidized pullulan (OPL) is constructed.
Chen Zhang, Surui Yang, Zhi Xu et al.· Journal of materials chemist...· 0 citations
Cerium molybdate-doped polyaniline nanoparticles are developed to enable a synergistic combination of photothermal therapy (PTT) and CDT, thereby triggering immunogenic cell death (ICD) and enhancing antitumor immunity and presenting a robust nanoplatform integrating chemodynamic and photothermal therapies for potent cancer immunotherapy.
Yulin Kuang, Cheng Lu, Bolan Yu et al.· Bioactive Materials· 0 citations