Open access
Aug 2026
Directed evolution of the bacteriophage endolysin PlyC reveals a charge-mediated mechanism of thermal stabilization
This validated directed evolution methodology can now be expanded to other endolysins and bacteriolytic enzymes for the purpose of increasing their thermal stability, thereby improving their practical utility as an antimicrobial biologic.
Ryan D. Heselpoth, Daniel C. Nelson
· Journal of Biological Chemis... · 0 citations