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Ryohei Kobayashi

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Open access Aug 2026

Engineering a highly active thermophilic F1-ATPase by homolog-guided exploration and machine-learning-assisted prioritization

The rotary motor F1-ATPase has been extensively studied as a model molecular machine, yet rational engineering of its catalytic activity remains challenging because ATP hydrolysis is regulated by long-range intersubunit allostery and large conformational transitions. Here, we developed a homolog-guided engineering stra...

Ryohei Kobayashi, K. Miyake, Tatsuya Oya et al. · 0 citations

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