Exploring the Substrate Selectivity of the Condensation Domain VibH through Engineered Adenylation Domains, Non-Native Amide Synthesis, and Substrate Recognition Analysis.
Nonribosomal peptide synthetases (NRPSs) are modular assembly-line enzymes that generate structurally diverse and biologically active natural products. Although adenylation (A) domain selectivity has been extensively characterized and engineered, the specificity of condensation (C) domains, which catalyze peptide bond...