Background Archival biobanks of formalin-fixed paraffin-embedded (FFPE) specimens represent a vast and underused resource for retrospective molecular studies linked to clinical follow-up. However, fixation-induced chemical modification and nucleic acid fragmentation limit scalable genomic and transcriptomic profiling....
Yi Niu, Qun-Zhi Xu, Chun-Yin Mak et al.· bioRxiv· 0 citations
The standard for long-term storage of tissue samples is formalin fixation and paraffin embedding (FFPE), a method that allows samples to be archived at room temperature for decades. However, RNA degrades during storage, and prolonged exposure to ~4% formaldehyde damages RNA and DNA, limiting the utility of RNA sequenci...
Elena Lill, Emily Dinh, Mehar Dhami et al.· Journal of Visualized Experi...· 0 citations
Global upregulation of transcription, or hypertranscription, is observed in most human cancers, driven by the action of oncogene-encoded transcription factors (TFs). TF interactions with RNA Polymerase II (Pol II) are mediated by kinases that phosphorylate the Pol II C-terminal domain (CTD) to drive productive elonga...
S. Henikoff, Robert N. Eisenman, K. Ahmad· Epigenetics & Chromatin· 0 citations
CDK12 facilitates transcriptional elongation and processivity by RNA polymerase II (Pol II). Although it has emerged as a promising actor and target in cancer, better understanding of CDK12 gene transcription control could inform the design of novel anti-cancer strategies. Here, we identify a co-dependency between CDK1...
Monika Mačáková, Cassidy Danyko, Stefan Oberlin et al.· bioRxiv· 0 citations
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