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There was no evidence of astrogliosis after microglia ablation as determined by proteome analysis and brain histology, crucial for interpreting changes to the CSF proteome in relation to neuroinflammatory changes in neurological diseases.
A reproducible human three-dimensional brain tissue model with neurons, astrocytes and microglia, replicating in-vivo-like maturation and enabling the study of Alzheimer’s disease-relevant perturbations and drug responses is developed.
It is shown that early complement associated pruning of inhibitory synapses precedes overt α-synuclein aggregation and neuronal loss in prodromal synucleinopathy, indicating disease-context dependent relationships between αSyn pathology and neurodegeneration.
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