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Challenges in modern microbiology: how to connect molecular insights obtained from laboratory model systems with the dynamic and complex responses of microbes in natural environments is highlighted.
These findings reveal remarkable architectural flexibility, mechanistic robustness, and a conserved mechanism in oriC, and highlight unwinding stability as a key determinant of oriC adaptation to rapid cell growth, providing insight into the evolution of oriC architecture.
In model bacteria, initiation of chromosome replication requires engagement of single-stranded DNA by oligomers of the DnaA-family initiator assembled within the origin DNA. Although arrays of double-strand motifs recognized by DnaA are a general feature of the origins, the DnaA-binding single-strand elements are eluci...
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