Open access
Aug 2026
Simplifying multiplex genome engineering in Saccharomyces cerevisiae with intron-mediated Random Assembly and INtegration (RAIN)
A nascent method for multiplex genome engineering in Saccharomyces cerevisiae that negates the requirement for cloning of expression cassettes is presented and the first reported use of intron-mediated assembly of promoters and ORFs for expression of a functional gene product is reported.
F. Harrison, Philip A. Kelso, Alexander C. Carpenter et al.
· FEMS Yeast Research · 0 citations