Author

Sang Woo Seo

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Open access Sep 2026

Programmable in vivo mRNA circularization for enhanced gene expression in bacteria

Abstract The minute-scale lifetime of mRNA strongly influences bacterial gene expression, whereas a robust and programmable approach to directly control the mRNA stability and topology remains elusive. Here, we develop CRESEnT (Circular RNA Expression for Stable and Enhanced Translation), a programmable in vivo mRNA ci...

Seongjun Park, G. Kim, Min Ha Kim et al. · Nucleic Acids Research · 0 citations

Review Open access Sep 2026

Electrochemical-biological hybrid conversion of captured carbon dioxide to bioproducts.

CO2 is a promising C1 feedstock for the sustainable production of fuels, chemicals, and materials, but its efficient conversion into value-added products remains challenging. Biological CO2 conversion provides high selectivity and access to structurally complex products under mild conditions, yet is limited by low prod...

Kim Junyeob, Lee Dayoon, H. Kim et al. · Bioresource Technology · 0 citations

2026

Construction of a Tl-CRISPRi Genetic Circuit in Bacteria for Translation-Level Gene Knockdown.

This chapter describes how to design spacer sequences and install them into the guide RNA expression plasmid and describes how to mutate the handle of gRNA to achieve tunable knockdown of a target gene.

G. Kim, H. Kim, Sang Woo Seo · Methods in molecular biology · 0 citations