Open access
Aug 2026
Specific cleavage of 5′ overhangs of non-target strand by Cas9 activated by target DNA binding
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has become a powerful genome-editing tool that uses RNA–DNA pairing to cleave target DNA with protospacer adjacent motif (PAM) sequences. While its primary function is well-studied, secondary activities remain poorly understood,...
Shaokang Lv, Qi Wang, Sheng-Jie Gao et al.
· Nucleic Acids Research · 0 citations