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Author

Shouling Xu

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Open access Sep 2026

Best practices for biotin ligase–based proximity labeling proteomics in plant systems

Abstract Proximity labeling (PL) proteomics, primarily powered by engineered biotin ligases such as BioID and TurboID, has emerged as a transformative approach for mapping protein association networks and subcellular proteomes in living cells. By covalently biotinylating proteins within a nanometer-scale radius of a ba...

Yi-Ling Fang, Jacob Moe-Lange, Shouling Xu et al. · 0 citations
Open access Jul 2025

Mapping the architecture of protein complexes in Arabidopsis using cross-linking mass spectrometry

Capturing molecular machines in action is essential for understanding protein complex architecture, cellular regulation, and gene function. Here, we present a large-scale structural proteomics resource for Arabidopsis thaliana generated using an optimized cross-linking mass spectrometry (XL-MS) workflow. Using the trif...

Cao Son Trinh, Ruben Shrestha, Peng-Zhi Mao et al. · 4 citations · ⚡2

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