Open access
Sep 2026
Functional mapping and engineering of the Sec translocon unlocked by a cell-free system
Almost all membrane proteins are inserted or translocated across membranes by the universally conserved Sec translocon. Despite its central role, experimental access to Sec function has remained limited. Here, we present ProSecCO (Protein Secretion in Cell-free via synthetic Operons), which is a cell-free protein synth...
M. Meier, Scott A. Scholz, Leo von Bank et al.
· Science Advances · 2 citations