Open access
Aug 2026
An engineered helper plasmid generates differential E4orf6 and L4-22/33K gene expression increasing AAV vector production
Helper plasmids that depend on native adenovirus gene expression have long been the standard for transient adeno-associated virus (AAV) production. Here, we demonstrate that engineering the required helper gene expression can greatly increase AAV production relative to the use of native adenovirus gene regulation. Two...
L. van Lieshout, Katrina Costa-Grant, Dimpal Lata et al.
· Gene Therapy · 0 citations