T. Dileepan

Durability and diversity of human upper airway T cell memory

Efficient detection of human antigen-specific CD4+ T cells with peptide-exchangeable HLA-DP4 tetramers and multiplexed magnetic enrichment.

The tremendous variation in human MHCII alleles is a barrier to using fluorophore-labeled peptide:MHCII tetramers to study antigen-specific CD4+ T cells. This is because matching of the MHCII molecule in the tetramer with each human subject is required. We show that fluorophore-labeled tetramers of peptides complexed t...

Abstract B084: Tracking tumor-specific CD4 T cells in pancreatic cancer reveals an essential role for cognate interactions with myeloid cells in remodeling the tumor microenvironment following immunotherapy

Our study identified an antigen (Ag)-specific CD4 T cell population in a preclinical orthotopic model of pancreatic ductal adenocarcinoma (PDA) that, upon expansion, elicited robust antitumor control. We developed an affinity-enhanced MHC-II tetramer to follow the endogenous tumor-specific CD4 T cells during pancreat...

Tumor-specific CD4 T cells cooperate with myeloid cells to remodel the pancreatic tumor microenvironment and enable effective immunotherapy

T tumor-specific CD4 T cells are central mediators of effective immunotherapy in solid tumors, and human immunotherapy transcriptomics shows conserved Th1 programming and Treg destabilization as a feature of response.