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Optimized protocols for tracing individual pollen tubes in Arabidopsis are presented and compared, offering valuable insights into reproductive signaling pathways that are relevant to both basic biology and applied breeding.
This study reveals that SWI3b promotes the initiation of megagametogenesis through chromatin-mediated repression of AGO5, leading to its exclusion from the MMC and thereby linking histone acetylation and epigenetic mechanisms to the development of the female germ cells.
A mechanism by which chromatin remodeling and m6A-mediated RNA regulation cooperate to suppress the precocious stability of key developmental regulators, thereby contributing to the establishment of the transcriptional program required for early embryo patterning in Arabidopsis is uncovered.
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