Breast cancer is the most prevalent cancer among women worldwide and a leading cause of cancer-related deaths. Piper aduncum is a tropical plant recognized for various potential medicinal properties, such as anti-breast cancer. This study aims to investigate the anti-breast cancer potential of bioactive compounds found in P. aduncum leaf ethanolic extract by measuring of cytotoxicity and using a computational approach. Analysis of cytotoxic using MTT assay. Information on active compounds was collected from the KNApSAcK database. Druglikeness, Pa, and Toxicity class were analyzed by using ADMET, Way2Drug, and Protox II. AutoDock vina was used to simulate the docking of chemicals with their protein targets. The cytotoxic analysis showed that the ethanolic extract of P. aduncum have IC50 values 105.75 μg/mL. P. aduncum contains 21 bioactive compounds. Methyl 2,2-dimethyl-8-(3'-methyl-2'-butenyl)-2H-1-chromene-6-carboxylate, 2,2-Dimethyl-8-prenylchromene 6-carboxylic acid, Methyl 2,2-dimethyl-2H-1-chromene-6-carboxylate, Anofinic acid, Asebogenin, Cardamomin were predicted to exhibit anti-breast cancer activities. Meanwhile, anofinic acid displayed the potential interaction with RAC1. In conclusion, as indicated by computational analysis, P. aduncum leaf has high potential as an anti-breast cancer agent by inhibiting protein RAC1.
Jantje Wiliem Souhaly, R. Pratiwi, L. Nugroho et al.· Jurnal Teknologi· 0 citations
Indonesian rice cultivars represent valuable genetic resources, yet many remain poorly characterized at the genomic level. Here, we generated 95.40 Gb of PacBio HiFi sequence data from seven Indonesian rice cultivars and constructed cultivar-specific consensus genomes using the telomere-to-telomere Nipponbare reference AGIS1.0. Sequencing coverage ranged from 27.92× to 41.58×, and the resulting consensus genomes spanned 387.93-390.54 Mb, with BUSCO completeness of approximately 98.3-98.5%. OrthoFinder assigned 99.1% of predicted proteins to 40,737 orthogroups, including 27,514 core orthogroups represented across all seven cultivars, indicating a highly conserved predicted gene space within the reference-guided framework. Targeted analysis recovered 278 of 280 cultivar-by-locus combinations representing 40 genes or gene family entries associated with grain pigmentation, nitrogen and amino-acid metabolism, and starch properties. Comparative predicted protein analysis prioritized ANS1, SBE2b, SSIIa/ALK, Wx/GBSSI, OsAAP6/qPC1, and SSI as candidates for further investigation. Among 269 completed AGIS1.0-anchored promoter comparisons, 159 passed quality-control criteria, whereas 110 were flagged for gene-model, boundary, synteny, or structural concerns. Notably, these flagged comparisons accounted for more than 90% of the alignment-derived sequence variation, emphasizing the importance of rigorous quality control when interpreting apparent promoter divergence. Collectively, these reference-guided genomic resources provide a standardized framework for investigating sequence variation in Indonesian rice germplasm and prioritize testable coding and regulatory candidates for functional validation and future genomics-assisted crop improvement.
Y. Purwestri, Adhityo Wicaksono, Siti Nurbaiti et al.· bioRxiv· 0 citations
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