Open access
Aug 2026
A Luciferase-Based Assay for Assessing Cap-Independent Translation in Wheat Germ Extract
A cell-free protocol to quantitatively assess cap-independent translation using wheat germ extract and a firefly luciferase (FLuc) reporter is described, suitable for testing combinations of IRES and CDS.
Max Cortot, Thorsten Stehlik, Aline Koch et al.
· Bio-protocol · 0 citations