Structural and biochemical basis for the formation of α-(1→3)-linked mannose residue in O-glycans of Saccharomyces cerevisiae.
The biosynthesis of O-mannose-type glycans in the budding yeast Saccharomyces cerevisiae requires the sequential action of multiple mannosyltransferases. However, the molecular mechanism governing α-(1→3)-linkage formation remains poorly understood. Mnt2p, a member of glycosyltransferase family 71 (GT71), catalyzes the...