Identifying resistance-conferring cytochrome P450s in weeds: experimental strategies and practical considerations.
Cytochrome P450 monooxygenases (P450s) play a pivotal role in metabolic herbicide resistance, yet demonstrating that a P450 can metabolize a herbicide is not sufficient to establish that it is responsible for resistance in weeds. The rapid expansion of transcriptomic resources and functional assays has accelerated the...