Simultaneous Site- and Residue-Specific Incorporation of Fluorinated Amino Acids for In-Cell Structural Biology by 19F Magic Angle Spinning NMR Spectroscopy
A 19F-labeling strategy is reported that combines site-specific incorporation of 4-(trifluoromethyl)-l-phenylalanine (tfmF) via genetic code expansion with fluorination of all tryptophan residues at the 7 position of the indole ring to expand the toolbox of 19F MAS NMR for cellular structural biology.