Theda Ulrike Patricia Bartolomaeus

Systematic quantification and removal of host DNA contamination in 16S rRNA gene sequencing.

16S ribosomal RNA (rRNA) gene sequencing is a standard tool for microbial community analysis. Challenges can occur, particularly in low-biomass samples, when host DNA triggers off-target amplification. Low-biomass microbiome studies are particularly vulnerable to contamination from host DNA, which can obscure microbial...