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Thorsten Stehlik

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Open access Aug 2026

A Luciferase-Based Assay for Assessing Cap-Independent Translation in Wheat Germ Extract

A cell-free protocol to quantitatively assess cap-independent translation using wheat germ extract and a firefly luciferase (FLuc) reporter is described, suitable for testing combinations of IRES and CDS.

Max Cortot, Thorsten Stehlik, Aline Koch et al. · 0 citations

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