DH-DeepLoc: fast, robust 3D nanoscale double-helix PSF localization via deep learning-MLE synergy
The double-helix point spread function (DH-PSF) is a crucial tool in three-dimensional single-molecule localization microscopy (3D-SMLM). DH-PSF-based data analysis typically follows a two-step "detection-localization" workflow, where the detection stage predominantly relies on template matching (TM) methods. However,...