Author

Tonya N. Zeczycki

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Open access Aug 2026

Amber Suppressor tRNA-Based Mutagenesis for Positional Semi-Saturated Mutagenesis with Natural Amino Acid Substitutions: An Approach for Mapping Positional Contributions to Protein Function

There is a growing interest in methods that illuminate the contributions of individual positions to a protein’s function by site-saturating mutagenesis. However, a commonly used approach for assessing variant libraries with deep mutational scanning relies on readouts of biological fitness, which is influenced by many p...

Pierce T. O'Neil, Tonya N. Zeczycki, Kyung-Tae Park et al. · Biophysica · 0 citations

Open access Jul 2026

Selective Reprogramming of Ribosome Assembly and Translational Control during Bacterial Growth Phase Transitions

A label-free proteomics workflow is applied to characterize protein-level differences in the Escherichia coli proteome between exponential and prolonged stationary growth phases, finding that multiple components of the translational machinery are selectively enriched during the exponential growth.

Manuel Terrazas-López, Vanessa Aitken, Tonya N. Zeczycki et al. · ACS Omega · 0 citations