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Author

V. McParland

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Open access Sep 2026

Systematic quantification and removal of host DNA contamination in 16S rRNA gene sequencing.

16S ribosomal RNA (rRNA) gene sequencing is a standard tool for microbial community analysis. Challenges can occur, particularly in low-biomass samples, when host DNA triggers off-target amplification. Low-biomass microbiome studies are particularly vulnerable to contamination from host DNA, which can obscure microbial...

T. Birkner, Theda Ulrike Patricia Bartolomaeus, V. McParland et al. · 0 citations

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