Melon (Cucumis melo L.) is an economically important horticultural crop in Indonesia, and its growth, yield, and fruit quality are strongly influenced by the balance between vegetative and reproductive growth. Excessive vegetative growth after fruit set can reduce fruit size and quality by competing with developing fruits for assimilates. Topping to regulate the number of leaves retained above the fruit may improve fruit development; however, information regarding the appropriate leaf retention level under hydroponic conditions remains limited, particularly for cultivars with contrasting leaf morphologies. [EA1.1][L1.2]This study evaluated whether broad- and narrow-leaf hydroponic melon cultivars respond differently to leaf retention level and identified a practical topping strategy for hydroponic melon production. Two cultivars, Sweet Hami (broad-leaf) and Dalmatian (narrow-leaf), were grown using a Dynamic Root Floating Technique (DRFT) system and subjected to four topping levels equivalent to retaining 8, 12, 16, and 20 leaves above the fruit. Sweet Hami showed a stronger response to leaf retention in terms of leaf area development and fruit weight, while Dalmatian exhibited a more gradual response. Retaining 16 leaves above the fruit resulted in favorable fruit weight and total soluble solids without excessive vegetative growth in both cultivars. These findings suggest that topping at the 17th internode may serve as a practical shoot management strategy for improving yield and fruit quality, while providing a useful reference for optimizing source-sink balance in hydroponic melon production across cultivars with different leaf morphologies.
Indonesian rice cultivars represent valuable genetic resources, yet many remain poorly characterized at the genomic level. Here, we generated 95.40 Gb of PacBio HiFi sequence data from seven Indonesian rice cultivars and constructed cultivar-specific consensus genomes using the telomere-to-telomere Nipponbare reference AGIS1.0. Sequencing coverage ranged from 27.92× to 41.58×, and the resulting consensus genomes spanned 387.93-390.54 Mb, with BUSCO completeness of approximately 98.3-98.5%. OrthoFinder assigned 99.1% of predicted proteins to 40,737 orthogroups, including 27,514 core orthogroups represented across all seven cultivars, indicating a highly conserved predicted gene space within the reference-guided framework. Targeted analysis recovered 278 of 280 cultivar-by-locus combinations representing 40 genes or gene family entries associated with grain pigmentation, nitrogen and amino-acid metabolism, and starch properties. Comparative predicted protein analysis prioritized ANS1, SBE2b, SSIIa/ALK, Wx/GBSSI, OsAAP6/qPC1, and SSI as candidates for further investigation. Among 269 completed AGIS1.0-anchored promoter comparisons, 159 passed quality-control criteria, whereas 110 were flagged for gene-model, boundary, synteny, or structural concerns. Notably, these flagged comparisons accounted for more than 90% of the alignment-derived sequence variation, emphasizing the importance of rigorous quality control when interpreting apparent promoter divergence. Collectively, these reference-guided genomic resources provide a standardized framework for investigating sequence variation in Indonesian rice germplasm and prioritize testable coding and regulatory candidates for functional validation and future genomics-assisted crop improvement.
Y. Purwestri, Adhityo Wicaksono, Siti Nurbaiti et al.· bioRxiv· 0 citations
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