Author

Vangelis Daskalakis

2 papers indexed here

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Open access Sep 2026

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains i...

Ilias G. Karvounis, Vangelis Daskalakis · Protein Science · 0 citations

Review Open access Aug 2026

A practical guide to investigating biomolecular condensates: a comment from the plant community.

This review aims to establish a foundation for rigorous, reproducible, and conceptually coherent research in condensate biology of plants and beyond, with emerging implications for crop genetic improvement and synthetic biology applications.

Jiaxuan Peng, J. Agudo-Canalejo, Monika Chodasiewicz et al. · Science China Life Sciences · 0 citations