HIGH-DIMENSIONAL MULTI-OMIC MAPPING OF POST-MORTEM HUMAN BRAIN USING ITERATIVE INDIRECT IMMUNOFLUORESCENCE IMAGING ON XENIUM-PROCESSED TISSUES
A post-spatial profiling workflow that combines the Xenium platform with iterative indirect immunofluorescence imaging (4i) on formalin-fixed paraffin-embedded (FFPE) human brain tissue to capture key pathological features for Alzheimer’s disease (AD) and facilitate cell segmentation is reported.