A target amplification-free CRISPR biosensor with digital microfluidic processing for microRNA profiling in AI-enhanced pediatric sepsis diagnosis.
MicroRNA signatures provide an informative readout of host-response dysregulation in pediatric sepsis, but their clinical translation remains limited by low target abundance and multiplexing requirements. Here, a target amplification-free CRISPR biosensing platform was established. The sensing mechanism couples Cas13a...