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Wen-Jie Chen

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Open access Sep 2026

One-pot RPA-CRISPR/Cas12a assay for rapid and sensitive detection of Sclerotium rolfsii in Glycine max

Soil-borne fungal diseases are major constraints to the yield of legume crops, and it is therefore urgent to develop rapid and accurate detection methods for plant pathogens. In this study, a one-pot fluorescent detection system combining recombinase polymerase amplification (RPA) with CRISPR/Cas12a was established f...

Wen-Jie Chen, Shu-Fang Chen, Xia-Yan Qin et al. · 0 citations

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