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Woosub Shin

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2026

Design of Conditional Guide RNAs for the Logical Regulation of Gene Expression.

This chapter describes the design of cgRNAs and provides detailed protocols for their in vivo characterization in E. coli, and shows how cgRNAs can be integrated into endogenous gene circuits to achieve sophisticated and logical regulation of gene expression.

Dongwon Park, Woosub Shin, Hansol Kang et al. · 0 citations