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Author

Xiayi Gong

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Open access Oct 2026

Accurate and Robust Measurement of Single-Molecule Fluorescence Spectral Heterogeneity

Understanding single-molecule fluorescence spectral heterogeneity (smFLUSH) is vital for developing new probes for multidimensional super-resolution microscopy and decoding nanoscopic environments. However, traditional analysis methods distort the data and fail to distinguish random background noise for actual spectral...

Xia-Yi Gong, Amrita Singh, Hong-Jing Mao et al. · 0 citations
Aug 2026

Spectroscopic DNA-PAINT for Simultaneous Multiplexed Super-Resolution Microscopy.

Simultaneous multiplexed super-resolution imaging remains a central challenge in single-molecule localization microscopy (SMLM), due to the limited number of spectrally distinguishable fluorophores and the tradeoff between spatial localization and spectral uncertainties. Here, we introduce spectroscopic DNA-PAINT (sDNA...

Md Abul Shahid, Shohag Chandra Das, K. Patel et al. · 0 citations

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