Multi-omics insights into choline chloride-enhanced methane production from waste activated sludge anaerobic digestion: linking rheological regulation with microbial metabolic responses.
Higher solids contents of waste activated sludge (WAS) improve the volumetric efficiency of anaerobic digestion but simultaneously reduce sludge flowability and substrate accessibility, leading to slower hydrolysis and methane production. Here, choline chloride (ChCl) was employed to overcome these constraints across 2...