Discovery of a thermostable eugenol oxidase for vanillin biosynthesis from vanillyl alcohol via resting whole-cell catalysis.
Bacterial eugenol oxidases (EUGOs) belong to the 4-phenol oxidase subfamily of the VAO/PCMH flavoprotein family and are versatile biocatalysts offering potential for use in biotechnology and organic synthesis. In this work, a thermostable EUGO from Capillimicrobium sp. (CsEUGO) was identified and overexpressed in E. co...