Open access
Aug 2026
Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins
ABSTRACT To overcome the labor‐intensive nature of traditional directed evolution, the promoted Escherichia coli‐assisted continuous evolution (PEACE) system has been developed. PEACE 1.0 employs a cytidine deaminase‑T7 RNA polymerase (T7 RNAP) fusion for targeted mutagenesis and integrates a toxin–antitoxin selection...
Xinyu Zhang, Zhan-Zhi Liu, Ying Xu et al.
· Advancement of science · 0 citations