Chilli veinal mottle virus (ChiVMV, Potyvirus capsivenamaculae) is an emerging potyvirus threatening tobacco production in China, but its transmission biology remains poorly understood. Here, we molecularly characterized a ChiVMV isolate based on the coat protein (CP) gene from tobacco collected in Huaping, Yunnan Province, China. The CP gene consisted of 861 nucleotides encoding 286 amino acids and shared 95.25% identity with global isolates. Phylogenetic analysis placed the isolate within the Yunnan clade. We systematically investigated the effects of acquisition access period (AAP), inoculation access period (IAP), vector density, and temperature on transmission efficiency by Myzus persicae. qPCR assays revealed that a 5 min AAP was sufficient to achieve a viral load comparable to the maximum, whereas extending the AAP to 20 min resulted in reduced acquisition. Viral load in aphids declined progressively after acquisition, with residual amounts at 0.5 h, 1 h, 12 h, and 24 h post-acquisition representing 37.5%, 21.3%, 5.6%, and 2.9% of the initial copy number per aphid, respectively. Transmission assays showed that the highest efficiency was achieved under the tested conditions with an AAP of 5 min, an IAP of 24 h, a density of 5 aphids per plant, and a temperature of 25 °C. A significant interference between AAP and IAP was also observed. These findings provide insights into ChiVMV epidemiology and establish standardized conditions for resistance screening and transmission studies.
Ning Jiang, Man-Wen Zhang, Yi-Bo Wang et al.· Agronomy· 0 citations
Chilli veinal mottle virus (ChiVMV, Potyvirus capsivenamaculae) can infect various Solanaceae crops, causing a decrease in yield and quality. In recent years, ChiVMV has become one of the most prevalent viruses affecting tobacco production, but its diversity and genetic structure are still elusive. In this study, 202 ChiVMV isolates were obtained from 13 tobacco-producing areas in Yunnan Province between 2021 and 2025. Analysis of the coat protein (CP) genes of the 202 isolates showed that there exist 193 haplotypes and abundant genetic diversity. The highest variation was observed at the N-terminus of ChiVMV CP. Further analysis displayed three high-confidence recombination events in the CP gene of ChiVMV Yunnan isolates, with isolate 25DLYP-7 from the Dali region playing a putative parental isolate for these recombinations. The results of neutral and selection pressure analyses indicated that the CP gene of ChiVMV Yunnan isolates exhibits purifying selection. Distribution analysis of these ChiVMV Yunnan isolates showed that geographical isolation is closely related to the genetic diversity of ChiVMV, and field weeds may serve as important initial sources of infection. Phylogenetic analysis demonstrated that ChiVMV Yunnan populations display close association with isolates from southwestern China, as well as isolates from South Asia and Europe.
Ning Jiang, H. Fang, Zhong-Yue Mai et al.· Viruses· 1 citation
It is indicated that healthy tobacco roots harbor disease-suppressive endophytic microorganisms and suggested that TM-1 may suppress F. solani by interfering with sugar transport and carbon acquisition and provide a potential biocontrol resource for the sustainable management of tobacco root rot.
Tian-Miao Li, Xiao-Yu Zhou, Fei Xiong et al.· Frontiers in Microbiology· 0 citations
