Remodeling the Active Site of Sucrose Phosphorylase for Effective Resveratrol Glycosylation.
The plasticity of the enzyme active site is widespread in naturally evolved homologous enzymes, but has been less explored in engineered enzymes. In this study, the plasticity of the active site in sucrose phosphorylases (SPs) for 3-O-α-glucopyranosyl-resveratrol synthesis was obtained. The mutant R135L/Y344Q of BlSP f...